Coding

Part:BBa_K5159001:Design

Designed by: Tianyue WU   Group: iGEM24_HKUST   (2024-09-20)


Schizochytrium sp. ATCC 20888 PUFA Synthase ORF_A with His-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 6719
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 206
    Illegal BglII site found at 2009
    Illegal BglII site found at 2069
    Illegal XhoI site found at 1414
    Illegal XhoI site found at 3445
    Illegal XhoI site found at 3688
    Illegal XhoI site found at 3772
    Illegal XhoI site found at 4105
    Illegal XhoI site found at 4341
    Illegal XhoI site found at 4354
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 121
    Illegal NgoMIV site found at 346
    Illegal NgoMIV site found at 946
    Illegal NgoMIV site found at 1366
    Illegal NgoMIV site found at 2083
    Illegal NgoMIV site found at 2107
    Illegal NgoMIV site found at 5581
    Illegal AgeI site found at 715
    Illegal AgeI site found at 1054
    Illegal AgeI site found at 7552
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1192
    Illegal BsaI site found at 2481
    Illegal BsaI site found at 6490
    Illegal BsaI site found at 7164
    Illegal BsaI site found at 7555
    Illegal BsaI.rc site found at 109
    Illegal BsaI.rc site found at 1561
    Illegal BsaI.rc site found at 2506
    Illegal BsaI.rc site found at 2536
    Illegal BsaI.rc site found at 2686
    Illegal BsaI.rc site found at 3415
    Illegal BsaI.rc site found at 3448
    Illegal SapI site found at 1809
    Illegal SapI site found at 2514
    Illegal SapI site found at 8082


Design Notes

We codon-optimized the sequence for expression in chassis Yarrowia lipolytica by referencing the organism's codon bias. To make the part RCF[10]-compatible, we also removed all EcoRI, XbaI, SpeI and PstI cut sites from the sequence by codon-optimization.


Source

This part comes from the genome of Schizochytrium sp. ATCC 20888. In our project, it was codon-optimized for Yarrowia lipolytica, divided into 7 fragments and synthesized by commercial provider Integrated DNA Technology (IDT)

References